Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice (2023)

Abstract

As has been shown in numerous studies, naturally occurring compounds can have protective effects towards mutagens and carcinogens. In the present study, the genotoxic/antigenotoxic effect of Toxicodendron quercifolium (poison ivy) extract, which has been identified as antigenotoxic in human HepG2 cells in former studies, was examined in the in vivo micronucleus assay using polychromatic erythrocytes (PCE) of bone marrow of CD1-mice. For this, D0 (1:10), D0 (1:25), D0 (1:50), D1 (1:50), D2 (1:50), and D4 (1:50) dilutions of ethanolic plant extract prepared on the basis of the "Hömoopathisches Arzneimittelbuch (HAB 2000)" were administered orally to CD1 mice over a period of two days. A significant increase (p<0.05) in micronucleus frequencies was found after administration of D0 (1:10), the highest tolerated dose. Additionally, antigenotoxic effects of T. quercifolium towards benzo(a)pyrene-induced micronucleus formation were studied. For that, four dilutions of the plant extract [D0, D2, D4, D6, each 1:50] were administered orally to CD1 mice for five days prior to the administration of benzo(a)pyrene (250 mg/kg b.w.) for another two days. It was found that the administration of the dilutions D0 (1:50) and D2 (1:50) of T. quercifolium extract significantly inhibited benzo(a)pyrene-induced micronucleus formation (p<0.0001). The results of this study indicated that T. quercifolium extract has the character of a so-called "Janus"-genotoxin: High doses led to a weak but significant increase of micronucleus frequencies whereas low doses showed chemopreventive effects towards benzo(a)pyrene-induced DNA damage. The constituents of T. quercifolium responsible for the genotoxic and antigenotoxic effects may be flavonoids, which are known to have prooxidative and scavenging effects and identified by HPLC-MS/MS.

Original languageEnglish (US)
Pages (from-to)1611-1617
Number of pages7
JournalFood and Chemical Toxicology
Volume42
Issue number10
DOIs
StatePublished - Oct 1 2004

Keywords

  • (Anti)genotoxicity
  • B(a)P, benzo(a)pyrene
  • In vivo mouse micronucleus assay
  • MNPCE, micronucleated polychromatic erythrocytes
  • NCE, normochromatic erythrocytes
  • NDI, nuclear division index
  • PCE, polychromatic erythrocytes
  • Toxicodendron quercifolium

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Mersch-Sundermann, V., Kassie, F., Böhmer, S., Lu, W. Q., Wohlfahrth, R., Sobel, R., Brunn, H. E., ElSohly, M. A., Ross, S. A., & Stahl, T. (2004). Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice. Food and Chemical Toxicology, 42(10), 1611-1617. https://doi.org/10.1016/j.fct.2004.05.006

Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice. / Mersch-Sundermann, Volker; Kassie, Fekadu; Böhmer, Sonja et al.

In: Food and Chemical Toxicology, Vol. 42, No. 10, 01.10.2004, p. 1611-1617.

Research output: Contribution to journalArticlepeer-review

Mersch-Sundermann, V, Kassie, F, Böhmer, S, Lu, WQ, Wohlfahrth, R, Sobel, R, Brunn, HE, ElSohly, MA, Ross, SA & Stahl, T 2004, 'Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice', Food and Chemical Toxicology, vol. 42, no. 10, pp. 1611-1617. https://doi.org/10.1016/j.fct.2004.05.006

Mersch-Sundermann V, Kassie F, Böhmer S, Lu WQ, Wohlfahrth R, Sobel R et al. Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice. Food and Chemical Toxicology. 2004 Oct 1;42(10):1611-1617. https://doi.org/10.1016/j.fct.2004.05.006

Mersch-Sundermann, Volker ; Kassie, Fekadu ; Böhmer, Sonja et al. / Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice. In: Food and Chemical Toxicology. 2004 ; Vol. 42, No. 10. pp. 1611-1617.

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title = "Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice",

abstract = "As has been shown in numerous studies, naturally occurring compounds can have protective effects towards mutagens and carcinogens. In the present study, the genotoxic/antigenotoxic effect of Toxicodendron quercifolium (poison ivy) extract, which has been identified as antigenotoxic in human HepG2 cells in former studies, was examined in the in vivo micronucleus assay using polychromatic erythrocytes (PCE) of bone marrow of CD1-mice. For this, D0 (1:10), D0 (1:25), D0 (1:50), D1 (1:50), D2 (1:50), and D4 (1:50) dilutions of ethanolic plant extract prepared on the basis of the {"}H{\"o}moopathisches Arzneimittelbuch (HAB 2000){"} were administered orally to CD1 mice over a period of two days. A significant increase (p<0.05) in micronucleus frequencies was found after administration of D0 (1:10), the highest tolerated dose. Additionally, antigenotoxic effects of T. quercifolium towards benzo(a)pyrene-induced micronucleus formation were studied. For that, four dilutions of the plant extract [D0, D2, D4, D6, each 1:50] were administered orally to CD1 mice for five days prior to the administration of benzo(a)pyrene (250 mg/kg b.w.) for another two days. It was found that the administration of the dilutions D0 (1:50) and D2 (1:50) of T. quercifolium extract significantly inhibited benzo(a)pyrene-induced micronucleus formation (p<0.0001). The results of this study indicated that T. quercifolium extract has the character of a so-called {"}Janus{"}-genotoxin: High doses led to a weak but significant increase of micronucleus frequencies whereas low doses showed chemopreventive effects towards benzo(a)pyrene-induced DNA damage. The constituents of T. quercifolium responsible for the genotoxic and antigenotoxic effects may be flavonoids, which are known to have prooxidative and scavenging effects and identified by HPLC-MS/MS.",

keywords = "(Anti)genotoxicity, B(a)P, benzo(a)pyrene, In vivo mouse micronucleus assay, MNPCE, micronucleated polychromatic erythrocytes, NCE, normochromatic erythrocytes, NDI, nuclear division index, PCE, polychromatic erythrocytes, Toxicodendron quercifolium",

author = "Volker Mersch-Sundermann and Fekadu Kassie and Sonja B{\"o}hmer and Lu, {Wen Qing} and Robert Wohlfahrth and Rosa Sobel and Brunn, {Hubertus E.} and ElSohly, {Mahmoud A.} and Ross, {Samir A.} and Thorsten Stahl",

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doi = "10.1016/j.fct.2004.05.006",

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pages = "1611--1617",

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TY - JOUR

T1 - Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice

AU - Mersch-Sundermann, Volker

AU - Kassie, Fekadu

AU - Böhmer, Sonja

AU - Lu, Wen Qing

AU - Wohlfahrth, Robert

AU - Sobel, Rosa

AU - Brunn, Hubertus E.

AU - ElSohly, Mahmoud A.

AU - Ross, Samir A.

AU - Stahl, Thorsten

PY - 2004/10/1

Y1 - 2004/10/1

N2 - As has been shown in numerous studies, naturally occurring compounds can have protective effects towards mutagens and carcinogens. In the present study, the genotoxic/antigenotoxic effect of Toxicodendron quercifolium (poison ivy) extract, which has been identified as antigenotoxic in human HepG2 cells in former studies, was examined in the in vivo micronucleus assay using polychromatic erythrocytes (PCE) of bone marrow of CD1-mice. For this, D0 (1:10), D0 (1:25), D0 (1:50), D1 (1:50), D2 (1:50), and D4 (1:50) dilutions of ethanolic plant extract prepared on the basis of the "Hömoopathisches Arzneimittelbuch (HAB 2000)" were administered orally to CD1 mice over a period of two days. A significant increase (p<0.05) in micronucleus frequencies was found after administration of D0 (1:10), the highest tolerated dose. Additionally, antigenotoxic effects of T. quercifolium towards benzo(a)pyrene-induced micronucleus formation were studied. For that, four dilutions of the plant extract [D0, D2, D4, D6, each 1:50] were administered orally to CD1 mice for five days prior to the administration of benzo(a)pyrene (250 mg/kg b.w.) for another two days. It was found that the administration of the dilutions D0 (1:50) and D2 (1:50) of T. quercifolium extract significantly inhibited benzo(a)pyrene-induced micronucleus formation (p<0.0001). The results of this study indicated that T. quercifolium extract has the character of a so-called "Janus"-genotoxin: High doses led to a weak but significant increase of micronucleus frequencies whereas low doses showed chemopreventive effects towards benzo(a)pyrene-induced DNA damage. The constituents of T. quercifolium responsible for the genotoxic and antigenotoxic effects may be flavonoids, which are known to have prooxidative and scavenging effects and identified by HPLC-MS/MS.

AB - As has been shown in numerous studies, naturally occurring compounds can have protective effects towards mutagens and carcinogens. In the present study, the genotoxic/antigenotoxic effect of Toxicodendron quercifolium (poison ivy) extract, which has been identified as antigenotoxic in human HepG2 cells in former studies, was examined in the in vivo micronucleus assay using polychromatic erythrocytes (PCE) of bone marrow of CD1-mice. For this, D0 (1:10), D0 (1:25), D0 (1:50), D1 (1:50), D2 (1:50), and D4 (1:50) dilutions of ethanolic plant extract prepared on the basis of the "Hömoopathisches Arzneimittelbuch (HAB 2000)" were administered orally to CD1 mice over a period of two days. A significant increase (p<0.05) in micronucleus frequencies was found after administration of D0 (1:10), the highest tolerated dose. Additionally, antigenotoxic effects of T. quercifolium towards benzo(a)pyrene-induced micronucleus formation were studied. For that, four dilutions of the plant extract [D0, D2, D4, D6, each 1:50] were administered orally to CD1 mice for five days prior to the administration of benzo(a)pyrene (250 mg/kg b.w.) for another two days. It was found that the administration of the dilutions D0 (1:50) and D2 (1:50) of T. quercifolium extract significantly inhibited benzo(a)pyrene-induced micronucleus formation (p<0.0001). The results of this study indicated that T. quercifolium extract has the character of a so-called "Janus"-genotoxin: High doses led to a weak but significant increase of micronucleus frequencies whereas low doses showed chemopreventive effects towards benzo(a)pyrene-induced DNA damage. The constituents of T. quercifolium responsible for the genotoxic and antigenotoxic effects may be flavonoids, which are known to have prooxidative and scavenging effects and identified by HPLC-MS/MS.

KW - (Anti)genotoxicity

KW - B(a)P, benzo(a)pyrene

KW - In vivo mouse micronucleus assay

KW - MNPCE, micronucleated polychromatic erythrocytes

KW - NCE, normochromatic erythrocytes

KW - NDI, nuclear division index

KW - PCE, polychromatic erythrocytes

KW - Toxicodendron quercifolium

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U2 - 10.1016/j.fct.2004.05.006

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JO - Food and Chemical Toxicology

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